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tmk [2019-04-15 18:31:25]
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tmk [2019-04-15 18:31:25]

thymidylate kinase
Locus
BSU00280
Isoelectric point
4.87
Molecular weight
23.73 kDa
Protein length
212 aa Sequence Blast
Gene length
639 bp Sequence Blast
Function
TTP biosynthesis
Product
thymidylate kinase
Essential
yes
E.C.
2.7.4.9
Synonyms
yaaP
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of pyrimidine nucleotides
  • Groups of genes → Essential genes

    • Gene

    Coordinates
    39,159 39,797

    Phenotypes of a mutant

  • essential PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + dTMP --> ADP + dTDP (according to UniProt)

    • Protein family

  • thymidylate kinase family (according to UniProt)

    • Structure

  • 2CCK (from Staphylococcus aureus, 53% identity, 74% similarity) PubMed

    • Localization

  • cytoplasm (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
    Description
    PubMed
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    Genes
    tmk-darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
    Description
    PubMed
    view in new tab

    Biological materials

    Expression vectors

  • pGP1689: expression of Strep-tmk in E. coli, in pGP172, available in Jörg Stülke's lab
  • pGP2671: expression of tmk-Strep, in pGP380 ,in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP2401 tmk-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab

    • References

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